Tag
slr2016 SS

Part:BBa_K125310:Design

Designed by: Krystle Salazar and Grace Kwan   Group: iGEM08_Hawaii   (2008-07-25)

slr2016 signal sequence from cyanobacterium Synechocystis; secretes protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The slr2106 signal sequence was synthesized as 4 different oligonucleotides that were then annealed and ligated together.

Primer name Primer Length G/C content Tm
slr2016-1_forward CTAGATGGCAGCAAAACAACTATGGAAAATTTTCAATC 38 34.2% 65.6C
slr2016-1_reverse GTCTAGGATTGAAAATTTTCCATAGTTGTTTTGCTGCCAT 40 35.0% 65.7C
slr2016-2_forward CTAGACCGATGAAGGGTGGAACTAGTAGCGGCCGCTGCA 39 59.0% 74.8C
slr2016-2_reverse GCGGCCGCTACTAGTTCCACCCTTCATCG 29 62.1% 70.5C

Source

The slr2016 signal sequence is located at the amino terminus of slr2016 polypeptides synthesized by Synechocystis sp. PCC 6803. The sequence is that identified by Sergeyenko and Los.

References

"Synechocystis sp. PCC6803 : slr2016." CYORF Cyanobacteria Gene Annotation Database . 17 Aug 2007. 08 July 2008. <http://cyano.genome.jp/cgi-bin/cyorf_view.cgi?ORG=syn&ACCESSION=slr2016>.

Sergeyenko, Tatiana V and Los, Dmitry A. "Cyanoabcterial leader peptides for protein secretion." FEMS Microbiology Letters 2006 (218): 351-357.